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2D Gel Information

#	Sample #	Sample identification and description	ul enclosed	Protein ug/ul	Protein dpm/ul	ul load
1
2
3
4
5

The protein concentration of the sample(s) was determined by methods of

The sample consists of:	complex mixture of polypeptides	purified polypeptides
	few polypeptides	don't know
	Is sample preparation necessary?	Yes No

What is the composition of the buffer you used to dissolve the sample?
What is the molecular wt. range of interest?
Is the sample radioactive?	Yes No	What is the isotope?
What is the total dpm froa all enclosed sample(s)?
What slab gel % acrylamide is described? (10% is standard)
What is This Isoelectric Point of most interest?

2D Gel Procedures:	2D Page (2D-ES-1)	Duplicate gels (2D-duplicate)
	Custom pH gradient (2D-ES-2)	2D with peptide slab (2D-ES-3)
	2D with 8% pH 8 (2D-ES-4)	2D MW down side (2D-ES-5)
	No Sulfhydral (2D-ES-6)	CA/CPK PI markers (2D-ES-7)
	Partial tubes gels (2D-ES-8)	Manual comparisons (2D-ES-9)
	Ltd Computer Comp (2D-ES-10)	Global Comp (2D-ES-11)
	2D Quant Few spots (2D-ES-12)	Laser Scanning (2D-ES-13)
	Electronic photos (2D-ES-14)	Mass Spec Fingerpr int (2D-ES-15)
	Internal AA sequencing (2D-ES-16)	N-term sequencing (2D-ES-17)
	LC-MS/MS (2D-ES-18)	Database Maintenance (2D-ES-19)
	Spot Cutout for seq (2D-ES-20)
1D Slab Gel Procedures:	SDS PAGE (1D-ES-1)	1D Quant (1D-ES-2)
	MW Distribution (1D-ES-3)	Double thickness (1D-ES-4)
	Peptide slab gels (1D-ES-5)	1D known protein det (1D-PKG-1)
	Assay Number:
Staining, Drying Procedures:	Coomassie blue stain (STP-1)	Silver stain (STP-2)
	Special silver stain (STP-3)	Ruby red stain (STP-4)
	Transparency drying (STP-5)	Paper drying (STP-6)
Radioactivity Procedures:	Autoradiography (RP-1)	Enhance treatment (RP-2)
	Fluorography (RP-3)	14C MW markers (RP-4)
	Send for identification of the phosphorylation site (RP-5)
Blotting Procedures:	PVDF blot (BP-1)	PVDF staining (BP-2)
	Nitrocellulose (BP-3)	India Ink Stain (BP-4)
	Colored MW markers (BP-5)	Western blotting (BP-6)
Sample Preparation Procedure and cGMP:	Protein determinations (SP-1)	TCA Precipitation (SP-2)
	Ethanol Precipitation (SP-3)	Nuclease Treatment (SP-4)
	Microdialysis (SP-5)	Lyophilization (SP-6)
	Add SDS + boiling (SP-7)	Custom sample prep (SP-8)
	GMP (cGMP-1)
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