Mouse Anti hepatitis delta virus (HDV)

Source mouse

Antigen: The hepatitis virus delta antigen-coding fragment (isolate TW2576) was inserted into pPinPoint plasmid and the expressed recombinant was used for mouse immunization. After cell fusion, the hybridomas obtained were screened for antibody reacting with the delta antigen fused in different format (MBP-25L) and tested negative for other fusion proteins.

Purification Method: Ion exchange column.

Antibody Formulation: HDV Antibody is lyophylized at a concentration of 1 g/ l from ammonium sulfate-precipitated ascites fluid ,PBS with 1% BSA.

Storage Procedure: Lyophilized: store at 4 C. After reconstitution, if not used within a month, aliquot and store at 20 C. If more dilute solution required, it is suggested that they be made in a buffered solution containing carrier protein such as PBS with 0.1% BSA.

Reconstitution: Reconstitute with 1 mg/ml of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

Stability / Shelf life: Store at 4C, stable for 6 months. For long-term storage, store at -20C

Applications 1. Immunofluorescence: Typical working dilution on frozen sections 1:200. 60 minutes primary antibody incubates at 25 C. 2. Western blotting: Recommended: working dilution on Western blotting 1:500. 60 minutes primary antibody incubates at 25 C.

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