IPTG (Isopropyl-β-D-Thiogalactopy

IPTG Isopropyl-β-D-Thiogalactopyranoside. An inducer of β-galactosidase used to promote the expression of proteins in cells controlled by the lac operator system. For blue/white screening, 0.1 mM final IPTG concentration in LB (Luria Broth) media is recommended. IPTG is a molecular mimic of allolactose. IPTG is an inducer for protein expression controlled by the Lac Operon. IPTG is often used in transformed E. coli colonies.

IPTG is commonly used with X-Gal for blue/white colony screening. IPTG is an analog of lactose that inactivates the lac repressor and induces synthesis of β-galactosidase. The expression of cloned genes, that are under the control of the lac operon, is induced by IPTG .

IPTG (Isopropyl-β-thio galactopyranoside) is white crystalline powder having Mol. wt.: 238.3, Purity: >99%.

Application:

IPTG is an inducer in cloning studies to allow maximum expression of genes cloned in expression vectors.
It helps in stimulating the production of β-galactosidase enzyme.
IPTG is a lactose analog which binds and inhibits the lac repressor and thereby strongly induces β-galactosidase production.

Technical Specifications:

Plant Cell Culture tested
Biotechnology Grade.
Dioxane free.

Composition:

99% by TLC (9:1 Butanol:Water) Rf= 0.5
White powder
Molecular weight 238.3

Usage

IPTG is intended for use in inducing expression from the lac promoter and its various derivatives.Use at a final concentration of 250-350 μg/mL in top agar and liquid cultures.Specific applications may require more or less IPTG .

General Specifications

Preparation of stock solution : Dissolve IPTG in water at 200 mg/ml. Sterilize solution through a 0.2 μm filter,dispense in small aliquots, and store at -20 °C. Such solutions usually are stablefor several months.

Molecular Weight : 238.31
Molecular Formula : C9H18O5S
CAS : 367-93-1
Storage : 2-8°C
Size : 25 g
Catalog No. : 21530057-3 ( 715052 )

Protocols

Gene expression of the lac operon using IPTG
IPTG Induction and Extraction of Proteins from Bacteria

Citations/References

Assembly and analysis of eukaryotic translation initiation complexes
AV Pisarev, A Unbehaun, CUT Hellen… - Methods in enzymology, 2007 - Elsevier ...
Isopropyl-β-d-thiogalactopyranoside (IPTG) was from Bioworld (Dublin, OH), GMP-PNP and Pefabloc were from Roche (Indianapolis, IN), protease inhibitor cocktail set III was from Calbiochem (La Jolla, CA), puromycin was from EMD Biosciences (San Diego, CA), and ...
In vitro reconstitution and biochemical characterization of translation initiation by internal ribosomal entry
VG Kolupaeva, S Breyne, TV Pestova… - Methods in enzymology, 2007 - Elsevier ... Louis, MO).
The protease inhibitor cocktail set III and puromycin are from Calbiochem (La Jolla, CA), GMP-PNP and Pefabloc are from Roche (Indianapolis, IN), and isopropyl-β-d-thiogalactopyranoside (IPTG) is from Bioworld (Dublin, OH). ...
Recent advances in the study of rTS proteins. rTS expression during growth and in response to thymidylate synthase inhibitors in human tumor cells
BJ Dolnick, K Lu, MB Yin… - Advances in Enzyme Regulation, 1997 - Elsevier ... MA).
Amiigase (thermally stable DNA ligase, EC 6.5.1.1) and gelase (agarase, EC 3.2.1.81) were obtained from Epicentre (Madison, WI). IsopropylthioDgalactoside (IPTG) was from BioWorld (Dublin, OH). Oli godeoxyribonucleotides ...
Artificial control of nitrate respiration through the lac promoter permits the assessment of oxygen-mediated posttranslational regulation of the nar operon in …
CE Noriega, V Sharma… - Journal of bacteriology, 2007 - Am Soc Microbiol
... The antibiotics (BioWorld, Dublin, OH) used for E. coli were as follows: ampicillin (100 µg/ml), gentamicin (15 µg/ml), and ... Intermediate levels of nitrate reductase activities can even be obtained using IPTG (isopropyl-ß-D-thiogalactopyranoside) (data not shown). ...
From no expression to high-level soluble expression in Escherichia coli by screening a library of the target proteins with randomized N-termini
KH Kim, JK Yang, GS Waldo… - METHODS IN …, 2008 - Springer
... 5. 1% (w/v) Agarose gel (Bioworld, Dublin, OH). 6. 12.5% (w/v) SDS-PAGE gel. ... Aliquot in 1 ml fractions and store at −20°C. 6. Isopropyl-β-D-thio-galactopyranoside (IPTG) stock solution (1.0 M): 4.8 g IPTG in 20 ml of sterile water. ..
Human {alpha}-Defensins Inhibit Hemolysis Mediated by Cholesterol-Dependent Cytolysins
RI Lehrer, G Jung, P Ruchala, W Wang… - Infection and …, 2009 - Am Soc Microbiol
... Cytotoxins. Recombinant LLO was purchased from bio-WORLD (Dublin, OH). ... ALO expression was induced by adding 0.5 mM isopropyl-β-D-1-thiogalactopyranoside (IPTG) when the cultures reached an optical density at 600 nm of 1.0 to 1.2. ...
Transcription regulation is demonstrated for five key enzymes in Giardia intestinalis cyst wall polysaccharide biosynthesis
AB Lopez, K Sener, EL Jarroll… - Molecular and biochemical …, 2003 - Elsevier
... expressed by diluting cultures, grown for 16 h in LB medium, 1:1 with fresh medium having a final IPTG concentration of 1 ... directed against GNA, AGM, and UAP were generated by Rockland (Gilbertsville, PA); the UAE antiserum was prepared by Bio-World (Dublin, OH). ...
Interaction of Proteus mirabilis urease apoenzyme and accessory proteins identified with yeast two-hybrid technology
SR Heimer… - Journal of Bacteriology, 2001 - Am Soc Microbiol
... at 37°C with aeration, induced in mid-exponential phase with 0.3 mM isopropyl- -D-thiogalactopyranoside (IPTG), and incubated for ... Purified UreC and UreD proteins embedded in SDS-polyacrylamide were supplied to BioWorld Laboratories (Dublin, Ohio) for the ...
Enhanced protein thermostability and temperature resistance
FT Robb… - US Patent App. 20,040/ …, 2003 -
... [0084] The Pfu-sHSP expression was induced by 1 mM isopropyl-1-thio-β-D-galacgtopyranoside (IPTG) for 3 hours. ... antibody preparations against the Pfu-sHSP were obtained by immunization of a rabbit with the purified, recombinant Pfu-sHSP (Bio-world, Dublin, Ohio). ...
BfpU, a soluble protein essential for type IV pilus biogenesis in enteropathogenic Escherichia coli
W Schreiber, KD Stone, MA Strong… - …, 2002 - Soc General Microbiol
... Ascites was prepared commercially (BioWorld, Dublin, OH) and purified using ... Bacteria were grown under BFP-inducing conditions and centrifuged (4000 g, 15 min at 4 °C). IPTG (1 mM) was added to the cultures for the last hour of incubation to induce ß-galactosidase ...
Identification of a phosphate regulatory site and a low affinity binding site for glucose 6-phosphate in the N-terminal half of human brain hexokinase
TY Fang, O Alechina, AE Aleshin, HJ Fromm… - Journal of Biological …, 1998 - ASBMB
... Isopropyl-1-thio-β-d-galactopyranoside (IPTG) was from BioWorld. ... are: Glu-6-P, glucose 6-phosphate; 1,5-AnG-6-P, 1,5-anhydroglucitol 6-phosphate; Glu-1,6-P 2 , glucose 1,6-bisphosphate; IPTG, isopropyl-1 ...
Identification of a truncated nucleoprotein in avian metapneumovirus-infected cells encoded by a second AUG, in-frame to the full-length gene
R Alvarez, BS Seal - Virology journal, 2005 -
... concentration of the supernatant fraction from infected cells was measured for protein concentration by Bradford's reagent (Bioworld, Dublin, OH) at 595 nm. ... Protein was induced with IPTG at 24 hours post-transfected and total proteins were ...
Nonaggregating mutant of recombinant human hexokinase I exhibits wild-type kinetics and rod-like conformations in solution
AE Aleshin, M Malfois, X Liu, CS Kim… - Biochemistry, 1999 - ACS Publications
... Isopropyl-1-thio-β-d-galactopyranoside (IPTG) was from BioWorld. ... stirring of 200 rpm and a filtered air flow of 6 L/min) at 37 °C to early log phase (A 600 = 0.4), after which isopropyl-d- thiogalactopyranoside (IPTG) was added to ... 1. Lowry, OH, and Passonneau, JV (1964) J. Biol. ...
Heterologous expression of functional Ptr ToxA
RP Tuori, TJ Wolpert… - Molecular plant-microbe …, 2000 - Am Phytopath Society
... used to inoculate 25 ml of fresh LBA and incubated with shaking at 37°C for 1 h. Expression of fusion proteins was induced by addition of isopropyl-β-D- thiogalactopyranoside (IPTG), to a ... Antiserum against refolded, purified NC-FP was raised at Bioworld (Dublin, OH). ...