Blue Sepharose® CL-6B

• A dye-ligand affinity matrix for purification of albumin, enzymes (including NAD⁺ and NADP⁺), coagulation factors, interferons and related proteins.
• The ligand, Cibacron Blue F3G-A, is covalently coupled to Sepharose® via chlorotriazine ring.
• Supplied as suspension in 20% ethanol.
• Due to a broad spectrum of the affinity, this product can be used as a general purpose protein purification tool.
  Reference:Clonis, Y.D., Atkinson, A., Bruton, C.J., and Lowe, C.R. (1987). "Reactive Dyes in Protein and Enzyme Technology." stockton Press, New York.

Technical Specifications

• Matrix: Highly cross-linked agarose, 6%
• Ligand: Cibacron Blue F3G-A
• Ligand coupling method: Triazine coupling
• Ligand density: 8µmol Cibacron Blue/ml
• Binding capacity: > 15mg bovine serum abumin/ml packed gel
• Particle size (range): 45-165µ
• pH stability: 3-13
• Coupling conditions: pH 9-10,
• Storage: 2-8ºC

Made with SEPHAROSE® chromatography media. SEPHAROSE® is a trademark of GE Healthcare Bio-Sciences AB (formerly known as Amersham Biosciences AB.

Protocol for the Purification of Wnt Proteins

J Chromatogr. 1984 Jan 20;283:199-210.

Blue Sepharose behaved lika a strong cationic ion exchanger because of the negative charges of its dye ligand, Cibacron Blue. A study of the protein-Cibacron Blue interactions by phase partition and equilibrium dialysis revealed the presence of high-affinity binding sites both in the case of the (di)nucleoide-dependent enzymes entirely of alpha-helix or beta-sheet.  Preparative applications of these are illustrated in terms of the optimization of affinity chromatography experiments.