CasPase™ 6, Assay Kit
Provides reagents for the detection of apoptosis by measuring the caspase activity - an early indicator of apoptosis. Assay can be monitored with either a fluorescence reader, a spectrophotometer or microtiter plate reader. Uses AFC-substrate which exhibits both fluorescence and absorbance spectral shifts between the substrate-conjugate and the free dye (AFC).
During the CasPase reaction, the substrate releases AFC free dye and undergoes both a fluorecence and absorbance shift. Since AFC-substrate exhibits dual-mode detection capability, arbitrary fluorescence values can be standardized to an optical density value. When measuring fluorescence, the assay reaction is excited at 390-400nm. If the assay is measured with a spectrophotometer or microtiter plate reader, the absorbance is measured at 390-400.
Specifications:
Assay can be conveniently adapted for high-throughput 96-well format.
Assay system may be used with purified enzyme preparations, cell extracts or tissue lysates.
Supplied with necessary assay buffers, enzyme specific AFC-substrate, free dye (AFC) and caspase inhibitor Z-VAD-FMK for establishing proper positive and negative controls and standards.
50 Assays
| Brand Name | : | BIOPLUS |
Size | : | 1 Kit |
Catalog No | : | 20710004-1 ( 526122 ) |
Model | : | 786-203A |
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