The BIO-WORLD Serum Abundant Protein Removal (SAPR ) kit (New Cat #2017-0000; Old cat#515911) removes four highly abundant proteins from human serum and plasma. These high-abundant proteins include albumin, haptoglobin, transferrin, and alpha-1-antitrypsin. The high affinity-based columns contain Separopore™ -matrix coupled with antibodies against these major serum proteins and include buffers. The SAPR kit is designed to provide specific, simultaneous, and complete removal of these four proteins, which comprise 80-90% of the proteins in human serum.
The presence of abundant proteins in human serum and plasma often masks the detection of low-abundant proteins of interest that serve as biomarkers for disease when being studied by 2D gel electrophoresis or LC/MS. After depletion of these high-abundant proteins, serum samples consist of an enriched pool of low-abundant proteins that can be further studied without significant interferences with abundant proteins.
The BIO-WORLD SAPR-HSA kit (catalog #515912) removes the highly abundant protein Human Serum Albumin only. This protein can also be removed by using Blue-Separopore™ (catalog #506480)