Recombinant Bovine Aprotinin (AP)

Source: Maize (Corn)

Background: Aprotinin inhibits the activity of several proteolytic enzymes such as chymotrypsin, kallikrein, plasmin and trypsin. It is present in blood and in most tissues, with a high concentration in lung, inhibits pro-inflammatory cytokine release and maintains glycoprotein homeostasis. In platelets, aprotinin reduces glycoprotein loss (e.g., GpIb, GpIIb/IIIa), while in granulocytes it prevents the expression of pro-inflammatory adhesive glycoproteins.

Description : Recombinant Bovine Aprotinin is approximately 6,512 Dalton highly purified and designed specifically for cell culture applications, protein purification, diagnostic testing and pharmaceuticals. It is produced without the use of animal- or human-derived materials and is therefore free from risk of contaminating human or animal-derived viruses or prions.

Physical Appearance: Sterile Filtered lyophilized powder.

Formulation: The protein was lyophilized without any additives.

Solubility: It is recommended to reconstitute the Aprotinin in sterile 18MO-cm H2O not less than 100 g/ml, which can then be further diluted to other aqueous solutions.

Stability: Aprotinin although stable at room temperature for 3 weeks, should be stored at 40C. Please do not freeze.

Purity: Greater than 90.0%, determind by Gel Electrophoresis.

Biological activity: 4.6 TIU/mg

Unit Defenition: One Trypsin Inhibitory Unit (TIU) is equal to the amount of inhibitor with the ability to inhibit two trypsin units by 50% where one trypsin unit will hydrolyze 1.0 mole of N-alpha-benzoyl-DL-Arginine-p-Nitroanilide (BAPNA) per minute at pH 7.8 at 250C.

Usage: BioWORLD's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.