Calpain-1 (mu-Calpain)

Source: Human Eryhtrocytes

Description: This protein consists of an 80-kDa large subunit and a 30 kDa small subunit. The protein was purified by sequential chromatography through DEAE-Sepharose, A1.5m Bio-Gel, and Phenyl-Sepharose® CL-4B columns.

Physical Appearance: Sterile Filtered colorless solution.

Formulation: 50mM imidazole-HCl, 100mM NaCl, 5mM EGTA, 1mM DTT and 10% sucrose.

Stability: -Calpain although stable at 100C for 1 week, should be stored desiccated below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Purity: Greater than 90% as determined by: Analysis by reducing and non-reducing SDS-PAGE Coomassie.

Applications: This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection. For adsorption we suggest the following procedure: A- Dilute 1 l of the antiserum against -calpain in 1ml of the usual buffer for immunohistochemistry (final dilution 1:1000). B- Add 1 g of protein to 1ml of the diluted antibody solution and mix well. C- Incubate for at least 6 hours in the cold. D- Apply to tissue-sections and incubate for 3 days. E - Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB). As a result, the immunostaining should be strongly reduced or even completely prevented.

Refrencesy: Lane et al. (1992) A comparison of the intracellular distribution of -calpain, mcalpain and calpastatin in proliferating human A431 cells. Exp. Cell Res. 203:5-16.

Usage : bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Storage	:	should be stored desiccated below -180°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Size	:	2 µg
Catalog No	:	22060000-1 ( 507362 )
Model	:	PRO- 363