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Human Interleukin-2 (Recombinant)

 

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Recombinant Human Interleukin-2

Source: E.Coli

Description: Recombinant Human IL-2 produced in E.Coli is a single, non-glycosylated mutein (variant form) of human IL-2 polypeptide chain containing 134 amino acids and having a molecular mass of 15517 Dalton. Our rHuIL-2 has an Ser substitute for Cysteine at position 126. The rHuIL-2 is purified by proprietary chromatographic techniques.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: The protein (1.1mg/ml) was lyophilized after extensive dialysis against 0.17mg sodium monobasic & 0.89mg dibasic sodium phosphate buffer to a pH=7.5.

Solubility: It is recommended to reconstitute the lyophilized IL-2 in sterile 20mM acetic acid (AcOH) not less than 100 g/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized rHuIL-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution rHuIL-2 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pro-Thr-Ser.

Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity: rHuIL-2 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of murine CTLL-2 cells is less then 0.0645 ng/ml, corresponding to a Specific Activity of 16.9 MIU/mg.

Endotoxin: Less than 0.1 ng/ g (IEU/ g) of HuIL-2.

Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.614 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of IL-2 as a Reference Standard.

Usage: BioWORLD's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.


Catalog No.:22060379-1 ( 508786 )
Size:10 μg
Brand Name:bioX
Hazmat Shipping:Non-hazardous
Shelf life:2 years
Storage:-20°C

*Cold Shipping and packing charges apply.

  Protocols

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