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Cytokeratin 8 Human (Recombinant)

 

MSRP: $189.46*

Price: $161.93*

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Synonyms

Keratin type II cytoskeletal 8, Cytokeratin-8, CK-8, Keratin-8, K8, KRT8, CYK8, KO, CK8, K2C8, CARD2.

 

Keratin 8 and 18 (K8/18) are the major components of intermediate filament (IF) proteins of simple or single-layered epithelia.

 

Description

Cytokeratin 8 Human Recombinant produced in E. coli is a single, non-glycosylated polypeptide chain having a molecular mass of 53,532 Dalton. The KRT8 is purified by proprietary chromatographic techniques.

 

Source

Escherichia coli

 

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

 

Formulation

The protein (1mg/ml) was lyophilized after from a sterile solution containing 30mM Tris-HCL pH-8, 9.5M urea, 2mM DTT, 2mM EDTA and 10mM methylammonium chloride.

 

Solubility

It is recommended to reconstitute the lyophilized KRT8 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

 

Stability

Lyophilized KRT8 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution KRT8 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

 

Reconstitution to filaments

Performed by mixing equimolar amounts of cytokeratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCI, 2 mM dithiothreitol, 10 mM Tris-HCI, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).

 

Usage

Bio-world products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

 

Related Products

KRT14 Human, KRT18 Human, KRT18 Human, His, KRT19 Human, KRT20 Human, KRT8 Human, GST

 


Catalog No.:22060303-1 ( 507194 )
Size:5 μg
Brand Name:bioX
Purity:Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Hazmat Shipping:Non-hazardous
Storage:-20°C

*Cold Shipping and packing charges apply.

  Protocols

Please check the protocols on www.biolabprotocols.com

If you have any protocols or usage recommendations on any of our products that to share with other scientists. Please feel free to submit them to us at info@biolabprotocols.com and we will be delighted to post them for you on www.biolabprotocols.com


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