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Calmodulin Separopore® 4B

 

MSRP: $148.23*

Price: $126.69*

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Applications:

Calmodulin-Separopore® 4B is used for affinity purification of calmodulin-binding proteins including calmodulin-regulated proteins in eukaryotic cells and recombinant proteins containing calmodulin-binding peptide fusion tag. Calmodulin binds proteins principally through their interactions with hydrophobic sites on its surface. These sites are exposed after a conformational change induced by the action of Ca2+ on separate Ca2+-binding site. The binding of enzymes may be enhanced if the enzyme substrate is present and enzyme substrate calmoldulin-Ca2+ complexes are particularly stable. Used for IP studies and bulk purification of CAM-binding proteins. 

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.


Catalog No.:20181001-1 ( 506460 )
Size:1 mL
Brand Name:bioPLUS™, Separopore®
Hazmat Shipping:Non-hazardous
Shelf life:2 years
Storage:2-8°C
Appearance Form:Suspension
Appearance Color:White
Matrix:Separopore® 4B (agarose beads, 4 %)
Matrix Activation:Cyanogen bromide
Matrix Attachment:Amino
Particle Size Range:53 - 180 μm
Spacer Arm:1 atom
Suspension:Supplied as suspension in 0.5M NaCl containing 0.02% thimerosal
pH Stability:4 - 9
Chemical Stability:Stable to all commonly used aqueous solutions
Physical Stability:Negligible volume variation due to changes in pH or ionic strength.
Ligand:Calmodulin (Bovine)

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  Protocols

Please check the protocols on www.biolabprotocols.com

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