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Calmodulin Separopore® 4B

 

MSRP: $502.68*

Price: $429.64*

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Applications:

Calmodulin-Separopore® 4B is used for affinity purification of calmodulin-binding proteins including calmodulin-regulated proteins in eukaryotic cells and recombinant proteins containing calmodulin-binding peptide fusion tag. Calmodulin binds proteins principally through their interactions with hydrophobic sites on its surface. These sites are exposed after a conformational change induced by the action of Ca2+ on separate Ca2+-binding site. The binding of enzymes may be enhanced if the enzyme substrate is present and enzyme substrate calmoldulin-Ca2+ complexes are particularly stable. Used for IP studies and bulk purification of CAM-binding proteins. 

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.


Catalog No.:20181001-3 ( 506462 )
Size:10 mL
Brand Name:bioPLUS™, Separopore®
Hazmat Shipping:Non-hazardous
Shelf life:2 years
Storage:2-8°C
Appearance Form:Suspension
Appearance Color:White
Matrix:Separopore® 4B (agarose beads, 4 %)
Matrix Activation:Cyanogen bromide
Matrix Attachment:Amino
Particle Size Range:53 - 180 μm
Spacer Arm:1 atom
Suspension:Supplied as suspension in 0.5M NaCl containing 0.02% thimerosal
pH Stability:4 - 9
Chemical Stability:Stable to all commonly used aqueous solutions
Physical Stability:Negligible volume variation due to changes in pH or ionic strength.
Ligand:Calmodulin (Bovine)

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  Protocols

Please check the protocols on www.biolabprotocols.com

If you have any protocols or usage recommendations on any of our products that to share with other scientists. Please feel free to submit them to us at info@biolabprotocols.com and we will be delighted to post them for you on www.biolabprotocols.com


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