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Streptavidin Separopore® 4B


MSRP: $330.96*

Price: $282.87*

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  • Affinity matrix with immobilized Streptavidin by CNBr activation coupling method.
  • The base matrix is a rigid, highly cross-linked beaded agarose with high chemical stability.
  • The immobilized streptavidin binds biotin and biotinylated substances and can be used for affinity chromatography applications.
  • The interaction between streptavidin and biotin is very strong and requires denaturating conditions for elution, which may destroy both the ligandand the sample.
  • Strptavidin-Separopore® 4B is extremely useful for exploiting either the strong interactions of biotin and streptavidin or the somewhat weaker interactions of 2-iminobiotin and streptavidin.
  • It can be used in the purification of antigens, where biotinylated antibodies are incubated with antigen. The biotinylated antibody-antigen complex binds to Streptavidin Separopore from which the antigen can be eluted.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

Catalog No.:20181037-2 ( 506914 )
Size:5 mL
Brand Name:bioPLUS™, Separopore®
Hazmat Shipping:Non-hazardous
Shelf life:2 years
Matrix:Separopore 4B (agarose beads, 4%)
Matrix Activation:Cyanogen bromide
Particle Size Range:53 -180 μm
Binding Capacity:>20 μg biotin / ml drained gel
Suspension:Supplied as suspension in buffered saline solution with 0.05% sodium azide.
pH Stability:3 - 10
Binding Wash Buffer:50 mM ammonium carbonate, 0.5 M NaCl, pH 10. Elution buffer: 50 mM ammonium acetate, 0.5 M NaCl, pH 4
Ligand:Native Streptavidin (from Streptomyces avidinii)

*Cold Shipping and packing charges apply.


Please check the protocols on www.biolabprotocols.com

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