The Meta DNA Isolation Kit is used to isolate inhibitor-free, fosmid cloning-ready DNA from unculturable or difficult-to-culture microbial species present in environmental water, soil, or compost samples. The kit isolates high-molecular-weight DNA that is randomly sheared (with fragment sizes approximately 40 kb), does not require size selection, and can be used directly for end-repair and construction of fosmid libraries, PCR amplification, or next-generation sequencing. The kit can also be used to prepare 16S rRNA metagenomic libraries for phylogenetic classification or species-abundance studies.
The procedure uses filtration technology (with 1.2-µm and 0.45-µm membranes provided) and enzymatic lysis (with Ready-Lyse™ Lysozyme and Proteinase K) to isolate metagenomic DNA from water (100 ml per extraction), soil (1 g per extraction), or compost (300 mg per extraction). The purified DNA is free from humic and fulvic acids.
|Catalog No.||:||10760015-1 ( 525158 )
||Pkg. Format/Yield||:||1 Kit
||Shelf life||:||1 year