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Meta DNA Isolation Kit

 

MSRP: $240.98*

Price: $205.97*

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The Meta DNA Isolation Kit is used to isolate inhibitor-free, fosmid cloning-ready DNA from unculturable or difficult-to-culture microbial species present in environmental water, soil, or compost samples. The kit isolates high-molecular-weight DNA that is randomly sheared (with fragment sizes approximately 40 kb), does not require size selection, and can be used directly for end-repair and construction of fosmid libraries, PCR amplification, or next-generation sequencing. The kit can also be used to prepare 16S rRNA metagenomic libraries for phylogenetic classification or species-abundance studies.

The procedure uses filtration technology (with 1.2-µm and 0.45-µm membranes provided) and enzymatic lysis (with Ready-Lyse™ Lysozyme and Proteinase K) to isolate metagenomic DNA from water (100 ml per extraction), soil (1 g per extraction), or compost (300 mg per extraction). The purified DNA is free from humic and fulvic acids.

Note: This kit requires a suitable filtration apparatus (e.g., MilliPore Cat. No. XX1004700, Nalgene Cat. No. 130-4045), which is not included.
Contains: Extraction buffer, Filter Wash Buffer, Meta-Lysis Solution (2X), MPC Protein Precipitation Reagent, Proteinase K, Fosmid Control DNA, RNase A, Ready-Lyse™ Lysozyme Solution, TE Buffer, 0.45-µm cellulose acetate filter membranes, 1.2-µm cellulose acetate filter membranes.


Catalog No.:10760015-1 ( 525158 )
Size:10 Prep(s)
Pkg. Format/Yield:1 Kit
Brand Name:bioPLUS™
Shelf life:1 year
Storage:-20°C

*Cold Shipping and packing charges apply.

  Protocols

Please check the protocols on www.biolabprotocols.com

If you have any protocols or usage recommendations on any of our products that to share with other scientists. Please feel free to submit them to us at info@biolabprotocols.com and we will be delighted to post them for you on www.biolabprotocols.com


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