Albumin is the component of plasma proteins, with mol. Wt 66kD. When tubular function decrease and get damaged, the albumin can not be sucked back fully, and this will cause the increase of albumin content in urine, the serve will form proteinuria. The determination of albumin content in the blood or urine is an important basis for the assessment of urine function, renal injury site identification, assessment of diabetes, hypertension and drugs on renal function damage.
Principle of the test:
Albumin kits are based on competitive method. Coat micro well plates with albumin antibody to form solid-phase antibody, in competitive inhibition reaction, a certain amount of solid phose antibody and enzyme labeled albumin and non-labeled antigen 9std or saples) suppress competitive inhibition reaction, after the reaction, it will form solid phose Ab-enzyne labeled albumin complex. Remove the unbound HRP Ab. Add substrate and detect absorbant value with 450nm wavelength.
Item Quantity Storage Condition
Albumin Microplate (12 x 8 wells) 96 wells 4°C
Albumin Standard 1 vial 4°C
10X Diluent N Concentrate 30 ml 4°C
Biotinylated Human Albumin Antibody 1 vial -20°C
100X HRP Conjugate 80 µL -20°C
Chromogen Substrate 8 mL 4°C
Stop Solution 12 mL 4°C
20X Wash Buffer Concentrate 2 x 30 mL 4°C
Sealing Tapes 3 N/A
Concentration of Standards: ug/ml - 0.1, 03, 1, 5 12 and 80