Glycogen is an inert carrier that increases the recovery of nucleic acids after alcohol precipitation. Insoluble in ethanol or isoproanol, glycogen traps the nucleic acids during precipitation and forms a visible pellet upon centrifugation. As an inert co-precipitant, glycogen efficiently recovers from dilute solutions DNA or RNA molecules ranging in size from small oligonucleotides to megabase molecules.
Glycogen is a highly purified branched chain carbohydrate that does not add exogenous nucleic acids to the sample as do other coprecipitants such as yeast RNA or tRNA. Bio-world Glycogen Solution, 20mg/ml, is free of host DNA and is functionally tested for DNA precipitation. It will not affect DNA/RNA concentration measurements determined by A260/280 ratios. At concentrations less than ~8 μg/μl, glycogen will not interfere with downstream applications including most enzymatic reactions or electrophoresis.
||Pkg. Format/Yield||:||2 x 0.5 mL
||Shelf life||:||1 year