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Alpha Amylase Purification And Assay Kit

 

MSRP: $236.14*

Price: $201.83*

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Using the bioWORLD’s α-amylase Purification and Assay kit the enzyme can be purified from any bacteria/fungal extract or exudate and purified to homogeneity by ammonium sulphate precipitation followed by affinity chromatography. The α-amylase adsorption to starch immobilized Separopore® 4B-CL (Agarose) allows α-amylase to bind to starch more tenaciously, and allows instant elution using 2% (w/v) dextrin. Electrophoresis analysis can confirm the purity of the enzyme. 

For extracellular enzyme, the exudate can be precipitated using 60% Ammonium Sulfate, dialyzed and applied to the Affinity Matrix. 

The Separopore®4B – Amylase Affinity Matrix is supplied in 10% ethanol. Wash the gel after packing in a column with 1x Phosphate Buffer (Extraction Buffer).


Catalog No.:10170003-1
Size:1 Kit
Brand Name:bioPLUS
Shelf life:2 years
Storage:-20°C

*Cold Shipping and packing charges apply.

  Protocols

Please check the protocols on www.biolabprotocols.com

If you have any protocols or usage recommendations on any of our products that to share with other scientists. Please feel free to submit them to us at info@biolabprotocols.com and we will be delighted to post them for you on www.biolabprotocols.com


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