Hybridization Cocktail, 45% Formamide is designed to optimize complementary base-pairing of DNA and RNA in Southern Blot, Northern Blot, Dot Blot, Colony and in situ hybridizations. Components of these cocktails stabilize the target and probe nucleic acids, lower melting temperatures, concentrate the nucleic acids in solution and lower background on the membrane or slides.
Reference: Unger E.R., Budgeon L.R., Meyerson D., Brigati D.J., Am. J. Surg. Pathol., 10(1): 1-8 (1986).
- 45% Formamide
- The hybridization solution contains 25ug/ml of sheared denatured DNA(from Salmon sperm) in the blend of salts and buffers.
|Catalog No.||:||10250001-1 ( 705485 )
||Shelf life||:||4 months