Protein-A Red Separopore® 4B-MB functions the same as conventional affinity beads, but have enhanced visibility due to red colored Separopore® macrobeads. The red colored beads help provide visual monitoring to avoid the accidental loss of beads during pull-down in immunoprecipitation (IP) procedures, thereby increasing efficiency and yield.Affinity matrix with immobilized Protein-A by CNBr activation coupling method.
- For immunoprecipitation and co-immunoprecipitation experiments.
- Reliable tool to purify monoclonal and polyclonal IgG from ascites, serum and cell culture supernatants.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
||Brand Name||:||bioPLUS™, Separopore®
||Matrix||:||Red Separopore® 4B-MB (agarose macrobeads, 4%)
||Matrix Activation||:||Cyanogen bromide
||Particle Size Range||:||150 - 265 μm
||Binding Capacity||:||~ 20mg human IgG / ml drained gel
||Suspension||:||Supplied as suspension in 0.5M NaCl, 0.02% sodium azide
||pH Stability||:||3 - 9 (for use), 2 - 10 (for cleaning)
||Ligand||:||Recombinant Protein-A (42 kDa)
||Molecular Weight Range||:||6 x 104 - 2 x 107