| Distributors | Register Now  |  
Shopping cart( 0 Items )Order Status  
 
Home
emailprintBook mark

GST-Tagged Recombinant Protein Purification OnePass™ Column 1 x 1 ml

 

MSRP: $455.50*

Price: $389.32*

Add to Cart: QTY:



Download COA

Applications:

OnePass™ GST-Tagged Recombinant Protein Purification column consists of Glutathione-Separopore® 4B-CL conveniently prepacked column cartridges for low pressure flow chromatography. This product provides a one step purification method and permits rapid, mild and highly selective purifications of proteins containing glutathione binding sequences. Glutathione-Separopore is prepared by covalently coupling glutathione to epoxy-activated highly crosslinked 4% agarose beads to form a stable thioether linkage. The coupling was optimized to give a high binding capacity and could be greater than 5 mg of Glutathione-S-transferase (GST) per ml of wet gel. Reduced glutathione is covalently linked to Separopore® 4B-CL for use in affinity purification of glutathione-S-transferase (GST) and GST-fused proteins. Bound GST–fusion proteins are easily displaced from the resin by elution with buffers containing reduced glutathione. Mild elution conditions preserve protein antigenicity and function. The OnePass™ prepacked Glutathione- Separopore® spin columns simply purification of GST-tagged proteins.

 

Technical specifications:

  • Ligand: Reduced glutathione (GSH)
  • Matrix: Separopore® 4B-CL (highly crosslinked agarose beads, 4%)
  • Average Particle size: 52 - 165 µm
  • Molecular weight range: 6 x 104 – 2 x 107
  • Matrix activation: Epoxy
  • Matrix spacer: 12 atoms
  • Ligand density: 5 – 10 mg GST / ml drained gel
  • Binding capacity: 8 mg recombinant GST-tagged protein / ml drained gel
  • pH stability: 3 – 12
  • Storage: 2 °C – 8 °C 

 

References:

  • pCold-GST vector: a novel cold-shock vector containing GST tag for soluble protein production. Protein Expr Purif. (2008) 62: 120-7.
  • Glutathione S-transferase can be used as a C-terminal, enzymatically active dimerization module for a recombinant protease inhibitor, and functionally secreted into the periplasm of Escherichia coli. Protein Sci. (1997) 6: 2180-7.
  • Vectors for Cu2+-inducible production of glutathione S-transferase-fusion proteins for single-step purification from yeast. Yeast. (1994) 10: 441-9.
  • Expression and mutagenesis of recombinant human and murine erythropoietins in Escherichia coli. Biochim Biophys Acta. (1995) 1261: 35-43.
  • GST fusion vector with caspase-6 cleavage site for removal of fusion tag during column purification. Biotechniques. (2005) 38: 360-364.
  • Bacterial production of recombinant human poly(ADP-ribose) glycohydrolase. Protein Expr Purif. (2011) 75: 230-5.
  • Expression of lipase-solubilized bovine liver microsomal cytochrome b5 in Escherichia coli as a glutathione S-transferase fusion protein (GST-cyt b5). Protein Expr Purif. (2006) 45: 352-8.
  • Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene. (1988) 67: 31-40.
  • An automated method for high-throughput protein purification applied to a comparison of His-tag and GST-tag affinity chromatography. BMC Biotechnol. (2003) 3: 12. 
  • A strategy for high-level expression of soluble and functional human interferon alpha as a GST-fusion protein in E. coli. Protein Eng Des Sel. (2007) 20: 201-9.

 

Related products

  • D-Alanine Separopore® 4B-CL (SKU # 20181063)
  • L-Aspartic Acid Separopore® 4B-CL (SKU # 20181067)
  • L-Glutamic Acid Separopore® 4B-CL (SKU # 20181068)
  • Glycine Separopore® 4B-CL (SKU # 20181065)
  • L-Histidine Separopore® 4B-CL (SKU # 20181069)
  • L-Lysine Separopore® 4B-CL (SKU # 20181070)
  • L-Phenylalanine Separopore® 4B-CL (SKU # 20181071)
  • Poly-L-Lysine Separopore® 4B-CL (SKU # 20181075)
  • Glutathione Separopore® 4B (SKU # 20181050)
  • Glutathione Separopore® 4B (Lyophilized) (SKU # 20181077)
  • Glutathione Separopore® 4B (Microbeads) (SKU # 20181044)
  • Glutathione Separopore® 4B-CL (Super Flow) (SKU # 20181088)
  • Glutathione Separopore® 6B-CL (High Resolution) (SKU # 20182013)

 

Usage : bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

 


Catalog No.:20170004-1
Size:15 mL
Pkg. Format/Yield:3 x 5 mL
Brand Name:bioPLUS™
Storage:2-8°C

*Cold Shipping and packing charges apply.

  Protocols

Please check the protocols on www.biolabprotocols.com

If you have any protocols or usage recommendations on any of our products that to share with other scientists. Please feel free to submit them to us at info@biolabprotocols.com and we will be delighted to post them for you on www.biolabprotocols.com


  References and Citations

Share your publication or characterization data with us.
Please contact us to post reference.
E-mail : Send email


  Customer also viewed the following products

Protein A Separopore® 4B Cartridge Protein A Separopore® 4B Cartridge

Protein A Separopore® 4B Affinity matrix with immobilized Protein-A (from staphylococcus aureus) by CNBr activation coupling method. For purification and/or fractionation of IgG subclasses. Relia... more

Amylose Separopore® 4B-CL OnePass™ Column (Divinyl Sulfone-Coupled) Amylose Separopore® 4B-CL OnePass™ Column (Divinyl Sulfone-Coupled)

Description: An affinity matrix used for the isolation of proteins fused to maltose-binding protein (MBP). Amylose is linked to Separopore® (Agarose) 4B-CL via divinyl sulfone coupling. Informat... more

Zerobind™;-H (Human) Zerobind™;-H (Human)

Zerobind™-H (Human) Zerobind™ affinity columns are designed to remove non-specific backgrounds from various polyclonal antibodies generally used for western blotting.  Passing the... more

Protein A Separopore® 4B OnePass™ Column Protein A Separopore® 4B OnePass™ Column

Protein A Separopore® 4B Affinity matrix with immobilized Protein-A (from staphylococcus aureus) by CNBr activation coupling method. For purification and/or fractionation of IgG subclasses. Relia... more

Ask a question

  • Tag

 
DMSO-Bio-Max™
Featured Items: