Calcium-Free Tyrode Solution, Sterile

NaCl (127 mM), KCl (5 mM), NaH2PO4 (1.8 mM), MgCl2 (1 mM), 25 mM NaHCO3, D-glucose (10 mM), pH 7.4 ± 0.2. Prepared in DI water and sterilized.

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