CHAPS 10%, Sterile

CHAPS (3-cholamidopropyl dimethylammonio 1-propanesulfonate) is a non-denaturing zwitterionic detergent for solubilizing membrane proteins and breaking protein-protein interactions. CHAPS is commonly used for protein solubilization in isoelectric focusing and two-dimensional electrophoresis, especially for non-denaturing (without urea) isoelectric focusing.

CHAPS is used at concentrations between 1-4% (v/v) that are generally used in an isoelectric focusing gel. A commonly used isoelectric focusing sample solution consists of 8 M urea, 4% CHAPS, 50mM DTT and 40 mM Tris. Its small micellar molecular weight (6150) allows it to be removed from samples by dialysis.

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