Citrate Buffer 0.5M, pH 8.0, Sterile

The citrate buffer is composed of Citric acid monohydrate (C₆H₈O₇ • H₂O, MW 210.14) and Trisodium citrate dehydrate (C₆H₅O₇Na₃ • 2H₂O, MW 294.12) mixed in a fixed ratio to attain the desired pH. As a conjugate base of a weak acid, citrate can perform as a buffering agent or acidity regulator, resisting changes in pH. May be used for antigen retrieval of tissue samples. The citrate solution is designed to break protein cross-links; thus, unmasking antigens and epitopes in formalin-fixed and paraffin embedded tissue sections, resulting in enhancing staining intensity of antibodies. Citrate has anticoagulant activity as a calcium chelator.

Technical Information:

citrate buffer (pK1): 3.13 (25°C, pH range: 2.2-6.5)

citrate buffer (pK2): 4.76 (25°C, pH range: 3.0-6.2)

citrate buffer (pK3): 6.4 (25°C, pH range: 5.5-7.2)

• PH Value: 8.0

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