GuHCL 6.0M

• A widely used protein denaturant
• A strong chaotropic agent used at high concentrations for denaturing native proteins and inactivating RNAses.
• 6M concentration in water.

Specifications:

Biotechnology Grade

A260: <0.03%
A230: <0.15
Lead: <0.0005%
Iron: <0.0005%
RNase/DNase Activity: None detected

References:

Mukhopadhyay, A., Inclusion bodies and purification of proteins in biologically active forms. Adv. Biochem. Eng. Biotechnol. 56, 61-109., (1997)

Levine, A.D., et al., High Level expression and Refolding of Mouse Interleukin 4 Synthesized in Escherichia coli. J. Biol. Chem. 270, 7445-7452, (1995)

Rudloph, R. and Lilie, H., In vitro folding of inclusion body proteins. FASEB J. 10, 49-56, (1996)

Naglak, T.J. and Wang, H.Y., Recovery of a foreign protein from the periplasm of Escherichia coli by chemical permeabilization. Enzyme Microb. Technol. 12, 603, (1990)

Marston, F.A.O. and Hartley, D.L., Solubilization of protein aggregates. Meth. Enzymol. 182, 264-276, (1990)

• Appearance: Liquid
• Boiling Point: 132.9 °C at 760 mmHg (Predicted)
• Density: 1.34 g/cm3 at 20 °C
• HS Code: 29252000
• Melting Point: 180-185 °C(lit.)
• RTECS Number: MF4300000