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Description

Hybridization Cocktail

  • Designed to optimize complementary base-pairing of DNA and RNA in Southern Blot, Northern Blot, Dot Blot, Colony and in situ hybridizations.
  • Components of these cocktails stabilize the target and probe nucleic acids, lower melting temperatures, concentrate the nucleic acids in solution, and lower background on the membrane or slides.
  • Solution is stable for 4 months after thawing. Avoid freeze-thaw cycles.

  • Specifications:

  • 50% Formamide
  • Hybridization solution contains 25υg/ml of sheared denatured DNA(from Salmon Sperm) in the blend of salts and buffers.
  • Ref: Unger E.R., Budgeon L.R., Meyerson D., Brigati D.J., Am. J. Surg. Pathol., 10(1): 1-8 (1986).

    Properties

    Shelf life

    2 years

    Storage Temperature

    -20°C

    ECCN #

    EAR99