Hypotonic Lysis Buffer

Hypotonic Lysis Buffer is a detergent-free, ready-to-use solution for the isolation of cytoplasmic proteins from cultured mammalian cells. Cell lysis and subsequent isolation of the crude cytoplasmic fraction by a simple centrifugation step is complete in less than 30 minutes. The protocol is compatible with a variety of downstream applications including Western Blotting and ELISA procedures as well as protein assays which are sensitive to the presence of detergents in the lysis buffer.

Specifications

Ready-to-Use solution

Compatible with a variety of downstream applications

Detergent-free solution

Simple protocol to isolate crude cytoplasmic extracts

How to Use

Resuspend the cell pellets in Hypotonic Lysis Buffer and incubate on ice for 15 minutes. Disrupt the cells by homogenization and pellet the cellular debris. The supernatant contains the crude cytoplasmic extract.