Pfu Reaction Buffer with MgSO4 10X

200 mM Tris-HCl (pH 8.8), 100 mM ammonium sulphate, 100 mM KCl, 1% (v/v) Triton X-100, 1 mg/ml BSA and 20 mM magnesium sulphate. Prepared in DI water and filtered through 0.22 µ filter. Shipped cold on blue ice, store at -20 C.