Phosphate Buffer 100mM, pH 7.0, Sterile

Phosphate buffer has a concentration of 100mM and a pH of 7.0. It contains a mixture of monobasic sodium dihydrogen phosphate and a dibasic sodium monohydrogen phosphate. Phosphates have a high buffering capacity and are soluble in water. They inhibit many enzymatic reactions and procedures of molecular cloning. Phosphates also precipitate in ethanol making it impossible to precipitate DNA and RNA from buffers that contain large amounts of phosphate ions.

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