TSS Broth, pH 6.5, Sterile

TSS solution is used in the one-step procedure for the preparation of competent Escherichia coli and allows transformation of cells without heat-shock. 1 x TSS is LB broth contains 10% (wt/vol) polyethylene glycol, 5% (vol/vol) dimethyl sulfoxide (DMSO), and 50 mM Mg²⁺ at pH 6.5. It is autoclaved. TSS transformation is not super-efficient, as compared to transformation with electroporation, but it is much easier. For applications that require a high efficiency (e.g. transformation with linear DNA), this method is not good enough.

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