Zymogram Developing Buffer 10X, pH 7.45

Product Description
This buffer contains 0.5M Tris-HCl, (pH 7.45), 2.0M sodium chloride, 50mM calcium chloride, 0.2% Brij®-35. Zymography is an electrophoretic technique, based on SDS-PAGE, that includes an enzyme substrate copolymerized with polyacrylamide gel. Samples are prepared in Zymogram Sample Buffer without boiling to preserve the structure and activity of the enzyme. Following electrophoresis, the SDS is removed from the gel by washing in Zymogram Renature Buffer that contains a non-ionic detergent. The gels are then equilibrated in Zymogram Developing Buffer, which contains the divalent metal cation required for enzymatic activity. The zymogram is subsequently stained (commonly with Amido Black or Coomassie Brilliant Blue), and areas of digestion appear as clear bands against a darkly stained background where the substrate has been degraded by the enzyme.

Warning: For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.

• Appearance: Liquid
• PH Value: 7.45