Cladosporium cladosporioides PCR Detection Kit

Norgen’s Cladosporium cladosporiodes PCR Detection Kit is a ready-to-use system for the isolation and detection of C. cladosporiodes from plant samples. First, the kit contains components for the rapid isolation of total DNA, including fungal DNA, from plant samples using spin-column chromatography based on Norgen’s proprietary resin (Nucleic Acid Isolation). Second, the kit contains C. cladosporiodes Master Mix and controls to allow for PCR Ampification. The amplified PCR products are then detected using agarose gel electrophoresis. Alternatively, detection can be performed based on real-time PCR using melt curves (Detection). Please see the flowchart to the right.

The C. cladosporioides Master Mix contains reagents and enzymes for the specific amplification of a 320 bp region of the fungal genome. In addition, Norgen’s Cladosporium cladosporiodes PCR Detection Kit contains a second heterologous amplification system to identify possible PCR inhibition and/or inadequate isolation. This kit is designed to allow for the testing of 24 samples. The Cladosporium cladosporiodes PCR Primer Set and Controls are also available separately for end-point PCR detection.

FEATURES AND BENEFITS

• Isolate High Quality, Inhibitor-Free DNA - Fungal DNA can be isolated from fungi growing on culture plates, from plant tissue and fruit
• Rapid Procedure - Isolate and detect C.cladosporioides in under 3 hours.
• Accuracy in Interpreting Results – The amplification bands are clearly distinct, and the melt curves do not overlap
• High Sensitivity and Specificity - The limit of detection is 1500 copies/50 mg of plant tissue
• Purchase Primer Set and Controls Separately - The C.cladosporioides Primer Set, as well as the positive control and negative control can be purchased separately

For research use only and NOT intended for In vitro-diagnostics.