Plasma/Serum HIV RT-PCR Detection Kit

Norgen’s Plasma/Serum HIV RT-PCR Detection Kit is a ready-to-use system for the isolation and detection of HIV. First, the kit contains components for the rapid isolation of total RNA, including viral DNA, from the plasma/serum samples using spin-column chromatography based on Norgen’s proprietary resin (Nucleic Acid Isolation). Second, the kit contains HIV Master Mix and controls to allow for RT-PCR Ampification. The amplified PCR products are then detected using agarose gel electrophoresis. Alternatively, detection can be performed based on real-time RT-PCR using melt curves (Detection). Please see the flowchart to the right.

The HIV Master Mix contains reagents and enzymes for the specific amplification of a 297 bp region of HIV. In addition, Norgen’s Plasma/Serum HIV RT-PCR Detection Kit contains a second heterologous amplification system to identify possible PCR inhibition and/or inadequate isolation. The kit is designed to allow for the testing of 12 samples. The HIV RT-PCR Primer Set and Controls are also available separately for end-point RT- PCR detection.

FEATURES AND BENEFITS

• Isolate High Quality, Inhibitor-Free RNA - Removal of highly concentrated salts, metabolic wastes and proteins provides high quality, concentrated viral RNA.
• Rapid Procedure - Isolate and detect HIV in under 3 hours.
• Accuracy in Interpreting Results – The amplification bands are clearly distinct, and the melt curves do not overlap.
• High Sensitivity and Specificity - The limit of detection is less than 1000 VP/mL of plasma/serum
• Purchase Primer Set and Controls Separately - The HIV Primer set as well as the positive control and negative control can be purchased separately

For research use only and NOT intended for In vitro-diagnostics.