Plasma/Serum VZV PCR Detection Kit

Norgen’s Plasma/Serum VZV PCR Detection Kit is a ready-to-use system for the isolation and detection of VZV. First, the kit contains components for the rapid isolation of total DNA, including viral DNA, from the plasma/serum samples using spin-column chromatography based on Norgen’s proprietary resin (Nucleic Acid Isolation). Second, the kit contains VZV Master Mix and controls to allow for PCR Ampification. The amplified PCR products are then detected using agarose gel electrophoresis. Alternatively, detection can be performed based on real-time PCR using melt curves (Detection). Please see the flowchart to the right.

The VZV Master Mix contains reagents and enzymes for the specific amplification of a 306 bp region of VZV. In addition, Norgen’s Plasma/Serum VZV PCR Detection Kit contains a second heterologous amplification system to identify possible PCR inhibition and/or inadequate isolation. The kit is designed to allow for the testing of 24 samples. The VZV PCR Primer Set and Controls are also available separately for end-point PCR detection

FEATURES AND BENEFITS

• Isolate High Quality, Inhibitor-Free DNA - Removal of highly concentrated salts, metabolic wastes and proteins provides high quality, concentrated viral DNA.
• Rapid Procedure - Isolate and detect VZV in under 3 hours.
• Accuracy in Interpreting Results – The amplification bands are clearly distinct, and the melt curves do not overlap.
• High Sensitivity and Specificity - The limit of detection is less than 200 VP/mL of plasma/serum.
• Purchase Primer Set and Controls Separately - The VZV primer set as well as the positive control and negative control can be purchased separately

This item ships via Dry Ice. Special shipping charges are required for this.

For research use only and NOT intended for In vitro-diagnostics.