Plum Pox Virus RT-PCR Detection Kit

Norgen’s Plum Pox Virus RT-PCR Detection Kit is a ready-to-use system for the isolation and detection of Plum Pox Virus from plant samples. First, the kit contains components for the rapid isolation of total RNA, including viral RNA, from plant samples using spin-column chromatography based on Norgen’s proprietary resin (Nucleic Acid Isolation). Second, the kit contains Plum Pox Virus Master Mix and controls to allow for PCR Ampification. The amplified PCR products are then detected using agarose gel electrophoresis. Alternatively, detection can be performed based on real-time PCR using melt curves and SYBR Green qRT-PCR to obtain Ct values using the Plum Pox Virus qRT-PCR Detection Kit (Detection). Please see the flowchart to the right.

The Plum Pox Virus Master Mix contains reagents and enzymes for the specific amplification of a 295 bp region of the viral genome. In addition, Norgen’s Plum Pox Virus PCR Detection Kit contains a second heterologous amplification system to identify possible PCR inhibition and/or inadequate isolations thereby eliminating false negatives. This kit is designed to allow for the testing of 24 samples. This kit is also available for quantitaive real-time PCR detection using SYRG Green (Cat# 33500). The Plum Pox Virus qRT-PCR Detection Kit is also available in a 96-well format for high throughput analysis. The Plum Pox Virus RT-PCR Primer Set and Controls and the Plum Pox Virus qRT-PCR Primer Set and Controls are also available separately for end-point PCR detection and real-time PCR detection, respectively,

FEATURES AND BENEFITS

• Isolate High Quality, Inhibitor-Free RNA - High quality viral RNA can be isolated from plant tissue and fruit
• Versatile Procedures Available - Two options are available for detection: end-point PCR using the Plum Pox Virus RT-PCR Detection Kit and quantitative real-time PCR using the Plum Pox Virus qRT-PCR Detection Kit. The Plum Pox Virus qRT-PCR Detection Kit is also available in a 96-well format for high-throughput analysis.
• Rapid Procedure - Isolate and detect Plum Pox Virus in under 3 hours.
• Accuracy in Interpreting Results – The amplification bands are clearly distinct, and the melt curves do not overlap
• High Sensitivity and Specificity - The limit of detection is1000 genome copies
• Purchase Primer Sets and Controls Separately - The Plum Pox Virus RT-PCR Primers, as well as the positive control and negative control can be purchased separately.

For research use only and NOT intended for In vitro-diagnostics.