Salmonella enterica PCR Detection Kit (Meat Product)

Norgen’s Salmonella enterica PCR Detection Kit (Meat Products) is a ready-to-use system for the isolation and detection of S. enterica. First, the kit contains media components for the enrichment of S. enterica from food samples. Second, the kit contains components for the rapid isolation of total DNA, including bacterial DNA, from the enrichment using spin-column chromatography based on Norgen’s proprietary resin (Nucleic Acid Isolation). Lastly, the kit contains S. enterica Master Mix and controls to allow for PCR Ampification. The amplified PCR products are then detected using agarose gel electrophoresis. Alternatively, detection can be performed based on real-time PCR using melt curves (Detection). Please see the flowchart to the right.

The S. enterica Master Mix contains reagents and enzymes for the specific amplification of a 336 bp region of the S. enterica genome. In addition, Norgen’s Salmonella enterica PCR Detection Kit (Meat Products) contains a second heterologous amplification system to identify possible PCR inhibition and/or inadequate isolation. This kit is designed to allow for the testing of 24 samples. The Salmonella enterica PCR Primer Set and Controls are also available separately for end-point PCR detection.

FEATURES AND BENEFITS

• Isolate High Quality, Inhibitor-Free DNA - Removal of highly concentrated salts, metabolic wastes and proteins provides high quality, concentrated DNA.
• Rapid Procedure - Isolate and detect S. enterica in under 3 hours after sample enrichment
• Accuracy in Interpreting Results – The amplification bands are clearly distinct, and the melt curves do not overlap
• High Sensitivity and Specificity - The limit of detection is 1000 cfu/mL of enriched samples
• Purchase Primer Set and Controls Separately - The S. enterica Primer Set, as well as the positive control and negative control can be purchased separately

For research use only and NOT intended for In vitro-diagnostics.