Urine-Based HIV RT-PCR Detection Kit

Norgen’s Urine-Based HIV RT-PCR Detection Kit is a ready-to-use system for the isolation and detection of HIV. First, the kit contains components for the rapid isolation of total RNA, including viral DNA, from the urine samples using spin-column chromatography based on Norgen’s proprietary resin (Nucleic Acid Isolation). Second, the kit contains HIV Master Mix and controls to allow for PCR Ampification. The amplified PCR products are then detected using agarose gel electrophoresis. Alternatively, detection can be performed based on real-time RT-PCR using melt curves (Detection). Please see the flowchart to the right.

The HIV Master Mix contains reagents and enzymes for the specific amplification of a 297 bp region of HIV. In addition, Norgen’s Urine-Based HIV RT-PCR Detection Kit contains a second heterologous amplification system to identify possible PCR inhibition and/or inadequate isolation. The kit is designed to allow for the testing of 24 samples. The HIV RT-PCR Primer Set and Controls are also available separately for end-point RT- PCR detection.

FEATURES AND BENEFITS

• Non Invasive, Non Infectious Sample Source – Urine is not infectious for most human pathogens.
• Isolate High Quality, Inhibitor-Free RNA - Removal of highly concentrated salts, metabolic wastes and proteins provides high quality, concentrated viral RNA.
• Rapid Procedure - Isolate and detect HIV in under 3 hours.
• Accuracy in Interpreting Results – The amplification bands are clearly distinct, and the melt curves do not overlap.
• High Sensitivity and Specificity - The limit of detection is less than 1000 VP/mL of urine
• Purchase Primer Set and Controls Separately - The HIV Primer set as well as the positive control and negative control can be purchased separately

For research use only and NOT intended for In vitro-diagnostics