Tail Lysis Buffer 2, 2X

20 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, 0.2 mg/ml Gelatin, 0.9% (v/v) NP-40 and 0.9% (v/v) Tween-20, pH 8.3 � 0.2. Prepared in DI water and filtered through 1um filter. Shipped at room temperature, store at 2-8 C.