Tail Lysis Buffer 2

10 mM Tris-HCl, 50 mM KCl, 2.5 mM MgCl2, 0.1 mg/ml Gelatin, 0.45% (v/v) NP-40 and 0.45% (v/v) Tween-20, pH 8.3 ± 0.2. Prepared in DI water and filtered through 1 µ filter. Shipped at room temperature, store at 2-8 C.