Protein G Coated Microplates

96-well Protein G coated microplates provide alternative methods for immobilizing antibodies for ELISA and other plate-based assay techniques. These plates are uniformly and stably coated with Protein G. They enable antibodies or pre-estabilished antibody-antigen complexes to be captured to wells of the polystyrene plate for subsequent detection in plate readers. The strategy is useful for a variety of assay situations, but it is generally not suitable for sandwich-type ELISA.

Specifications:

• Retain antibody activity, which can be lost when antibodies are immobilized by passive adsorption
• Orient antibodies for maximum antigen-binding capacity
• Immobilize antibodies without prior purification
• Ensure minimal variation (<5% well-to-well) from consistent coating
• Reduce nonspecific binding because plates are pre-blocked
• Binds up to 2.5µg antibody/well (100µL coating volume)