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Description

Separopore® 4B Anti-Mouse IgG (FC) Antibody (Polyclonal IgG) (Agarose)

Cat# 20181091


Details

Host: Goat

Isotype: IgG

Class: Polyclonal

Immunogen: Mouse IgG (FC)

Applications:

Goat (anti-Mouse) IgG Separopore® 4B is used for immunoprecipitation and Co-immunoprecipitation studies. It is suitable for antibody purification or to remove unwanted cross-reactivity. Affinity isolated antibody is coupled to cyanogen bromide-activated Separopore® 4B. The antibody preparation is specific for the Fc fragment of mouse IgG when tested against purified mouse IgA, IgG, IgM, Fc and kappa and lamda light chains. Affinity purified goat anti-mouse IgG coupled to Separopore® offers the advantage of increased sensitivity for mouse IgG without cross-reactivity to other substances present on the membrane or cell surface.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

References

Epitope-tagged gonadotropin-releasing hormone receptors heterologously-expressed in mammalian (COS-1) and insect (Sf9) cells. Mol Cell Endocrinol. (1997) 127: 143-54.
Fc-receptor-mediated targeting of antibody-bearing liposomes containing dideoxycytidine triphosphate to human monocyte/macrophages. J Pharm Pharmacol. (1993) 45: 48-53.
Growth of rat-mouse hybridoma cells in immunosuppressed hamsters. An easy and effective method to prepare monoclonal antibodies from heterohybridoma cell lines. J Immunol Methods. (1990) 130: 25-31.
Evidence for interaction of human anti-idiotypic antibodies with CA 125 determination in a patient after radioimmunodetection. Clin Chem. (1990) 36: 164-7.
Monoclonal antibodies for affinity purification of IL-6/IFN- beta 2 and for neutralization of HGF activity. Hybridoma. (1989) 8: 561-7.
Protein A vectorized toxins--II. Preparation and \"in vitro\" cytotoxic effect of protein A-ricin A chain conjugate on antibody coated human tumour cells. Mol Immunol. (1988) 25: 473-7.
The human B cell-associated antigen CD24 is a single chain sialoglycoprotein. J Immunol. (1986) 136: 3779-84.
Purification of functional, determinant-specific, idiotype-bearing murine T cells. J Exp Med. (1978) 148: 915-24.

Properties

Shelf life

1 year

Storage Temperature

2-8°C

ECCN #

EAR99

Concentration

25 mg Separopore® per mL of buffer

Matrix

Separopore® 4B (agarose beads, 4%)

Particle Size Range

52 - 165 μm

Matrix Activation

Cyanogen bromide

Binding Capacity

0.4 mg / ml drained gel

Suspension/Column/Cartridge

Supplied as 50% slurry in PBS pH 7.4, 0.02% sodium azide

pH Stability

4 - 9 (for use), 2 - 10 (for cleaning)

Flow Specifications

70 - 140 cm / h

Ligand Density

> 5 mg IgG / ml drained gel

Molecular Weight Range

6 x 104 - 2 x 107