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Description

Separopore® Anti Bovine Albumin (BSA) Antibody (Polyclonal IgG)

Details

Host: Rabbit

Isotype: IgG

Class: Polyclonal

Immunogen: Bovine Albumin (BSA)

Application:

Anti-BSA-Separopore® 4B is used for immunoprecipitation and immunoaffinity purification of bovine serum albumin (BSA) from solutions (and co-immunoprecipitation of biomolecules that bind BSA). It is also used to remove BSA from solutions.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

References

References:

Production of antibodies that recognize specific tyrosine-phosphorylated peptides. Curr Protoc Immunol. (2002) 11: Unit 11.6.
Production of antibodies that recognize specific tyrosine-phosphorylated peptides. Curr Protoc Mol Biol. (2001) 18:Unit 18.6.
Growth suppressing factor for endothelial cells exhibits tumor regressing activity. Biol Pharm Bull. (1999) 22: 353-9.
Purification of a growth-suppressing factor for bovine artery endothelial cells from mouse lymphoma P388D1 cells. Biol Pharm Bull. (1999) 22: 16-20.
Characterization of a 180 kDa molecule apparently reactive with recombinant L-selectin. Glycoconj J. (1997) 14: 321-30.
Receptors on immunocompetent cells. 3. Specificity and nature of receptors on dinitrophenylated guinea pig albumin- 125I-binding cells of immunized guinea pigs. J Exp Med. (1971) 134: 517-31.

Properties

Shelf life

1 year

Storage Temperature

2-8°C

ECCN #

EAR99

Matrix

Separopore® 4B (agarose beads 4%)

Particle Size Range

52 - 165 μm

Matrix Activation

Cyanogen bromide

Binding Capacity

1.5 - 3.0 mg BSA / ml drained gel

Suspension/Column/Cartridge

Supplied as 50% suspension in 0.01M sodium phosphate (pH 7) and 15 – 20 mM sodium azide.

pH Stability

4 - 9

Flow Specifications

70 - 140 cm / h

Molecular Weight Range

6 x 104 - 2 x 107

Extent of Coupling

2.0 - 2.4 mg antibody / ml drained gel