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Description

Red Separopore® Anti-Bovine Albumin (BSA) Antibody (Polyclonal IgG) (Agarose)

Host: Rabbit

Isotype: IgG

Class: Polyclonal

Immunogen: Bovine Albumin (BSA)

Applications:

Anti-BSA ViewIP red Separopore® 4B is used for the purification of bovine serum albumin (BSA) and BSA-associated biomolecules or for removal of BSA from solutions. It is also used for immunoprecipitation and immunoaffinity purification of BSA from solutions. The red color makes it easy to track the pellet in immunoprecipitation and co-immunoprecipitation experiments.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

References

References:
Production of antibodies that recognize specific tyrosine-phosphorylated peptides. Curr Protoc Immunol. (2002)11: Unit 11.6.
Production of antibodies that recognize specific tyrosine-phosphorylated peptides. Curr Protoc Mol Biol. (2001)18:Unit 18.6.
Growth suppressing factor for endothelial cells exhibits tumor regressing activity. Biol Pharm Bull. (1999) 22: 353-9.
Purification of a growth-suppressing factor for bovine artery endothelial cells from mouse lymphoma P388D1 cells. Biol Pharm Bull. (1999) 22: 16-20.
Characterization of a 180 kDa molecule apparently reactive with recombinant L-selectin. Glycoconj J. (1997) 14: 321-30.
Receptors on immunocompetent cells. 3. Specificity and nature of receptors on dinitrophenylated guinea pig albumin- 125I-binding cells of immunized guinea pigs. J Exp Med. (1971) 134: 517-31.

Properties

Shelf life

1 year

Storage Temperature

2-8°C

ECCN #

EAR99

Matrix

Separopore® 4B (agarose beads, 4 %)

Particle Size Range

52 - 165 μm

Matrix Activation

Cyanogen bromide

Binding Capacity

1.5 - 3.0 mg / ml (bovine serum albumin)

Suspension/Column/Cartridge

Supplied as a 50% suspension in 0.01M sodium phosphate (pH 7) and 0.02% sodium azide

pH Stability

4-9 (for use), 2-10 (for cleaning)

Flow Specifications

70 - 140 cm / h

Ligand Density

2.0 - 2.4 mg antibody per ml settled resin