Cell Lysis Buffer IV for ChIP

50 mM HEPES-KOH, pH 7.5, 140 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% SDS, 1mM PMSF, pH 7.5. Prepared in DI water and filtered through 0.22 � filter. Shipped at room temperatur, store at 2-8 C.