TAE Buffer 25X, Liquid Concentrate

Product Description
TAE (Tris-Acetate-EDTA) buffer liquid concentrate has a 25X concentration and has a pH of 8.3 � 0.1. A concentration of 1X contains 0.04M Tris acetate and 0.001M EDTA. TAE is used for electrophoresis of nucleic acids in agarose and polyacrylamide gels. It can be used for both genomic and large supercoiled DNA, and can also be used as both a running buffer and a gel preparation buffer. It is recommended for resolution of RNA and DNA fragments larger than 1500 bp, for genomic DNA and for large supercoiled DNA. TAE buffer has a relatively low buffering capacity and therefore replacement of the buffer during prolonged electrophoresis is recommended.

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