PBS 1X with Saponin

Posphate buffered saline (1mM Na2HPO4, 137mM NaCl, 1.5mM KH2PO4, 2.7mM KCl)containing Saponin (1%), pH 7.4 +/- 0.2. Prepared in DI water and was filtered using 0.22 micron filter.