ABTS Diammonium Salt

ABTS Diammonium Salt, or 2,2'-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid. It is commonly used in ELISA procedures to determine the bonding between molecules. ABTS is also used as a substrate to detect the amount of Hydrogen Peroxide in a sample via spectrophotometry.

When used as a peroxidase substrate, ABTS diammonium salt produces a green end product that can be read at 405 nm. It can be used to detect antioxidant activity when oxidized with Potassium persulfate.

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References

See "Antioxidant activity applying an improved ABTS radical cation decolorization assay" on PubMed.