TPCK

A serine/cysteine protease inhibitor, which acts as an alkylating agent that interferes with translation

• Also known as N-Tosyl-L-lysine Chloromethyl Ketone Hydrochloride or 1-Chloro-3-tosylamido-4-phenyl-2-butanone.

Inhibition Spectrum:

• Irreversible inhibitor of chymotrypsin.
• Also inhibits some proteases cleaving substartes with a Phe at position P1 and some cysteine proteases such as papain, bromelain or ficin. Does not inhibit trypsin and zymogens.

Mechanism of Action:

• Requires an active enzyme and forms a covalent bond with His of the active site.
• Does not react with zymogens so addition of TPCK in a crude extract inactivates only active protease.
• Does not react with protease-inhibitor complexes.

Synonym: Tos-L-Phe-CMK; Tosyl-L-Phenylalanine chloromethylketone

• IUPAC_Name: N-[(2S)-4-Chloro-3-oxo-1-phenylbutan-2-yl]-4-methylbenzenesulfonamide
• RTECS Number: XT5613500