Agaricus bisporus Lectin (ABA/ABL) - HRP (Horseradish Peroxidase)

Agaricus bisporus Lectin (ABA/ABL) is isolated from edible mushroom and purified by using GalNAc Sepharose 4B. This Lectin is composed of four subunits with slightly different amino acid sequences and glycosylation specificity. ABA/ABL Lectin has high affinity for galactose-1,3-N-acetylgalactosamine (Gal-1,3-GalNAc; also known as T-antigen disaccharide) and galactose-1,3-N acetylglucosamine, but does not bind to monosaccharides. ABA is a potent mitogen for T cells and binds to serum protein IgA. Agaricus bisporus has an anti-proliferative effect on cancerous cells. ABA can be internalized by clathrin-coated vesicles after binding to surface glycoproteins, thus making ABA an inhibitor of its nuclear import of signal-dependent protein. Horseradish peroxidase (HRP) is a 40 kDa protein that catalyzes the oxidation of substrates by hydrogen peroxide, resulting in a colored or fluorescent product or release of light as a byproduct of the reaction. It is mostly used for blotting, immunoassays and immunohistochemistry methods. Agaricus bisporus Lectin (ABA) is conjugated to H.

Applications: Glycobiology, Immunofluorescence, Immunohistochemistry, Immunocytochemistry

Recommended usage: Recommended concentrations from 0.5-10 ug/ml in 1XPBS.

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Storage Instruction: To prevent degradation and avoid repeated freeze-thaw cycles, aliquot your product into small, single-use volumes and store them at -20°C

Application

Blotting, ELISAs, Immunohistochemistry, Glycobiology