Allium sativum Lectin (ASA) - HRP (Horseradish Peroxidase)

Allium sativum lectin (ASA) is isolated from garlic and purified with affinity chromatography. It is a dimer of two subunits. ASA binds to several α1-2-linked mannose residues. The lectin recognizes internal mannose and binds to the core pentasaccharide of N-linked glycans. In addition, the removal of sialic acids enhances binding activity.  ASA has shown antiproliferative and apoptosis-inducing activity, making it a relative subject in cancer research.

Horseradish peroxidase (HRP) is a 40 kDa protein that catalyzes the oxidation of substrates by hydrogen peroxide, resulting in a colored or fluorescent product or release of light as a byproduct of the reaction. It is most commonly used for blotting, immunoassays and immunohistochemistry methods. Allium sativum Lectin (ASA) is conjugated to HRP at an appropriate ratio to provide optimal staining characteristics.

Recommended Usage: Recommended dilution ranges 0.5 – 10 µg/ml. 1XPBS can be used for reconstitution. Less than 0.5 ug /ml will agglutinate rabbit erythrocytes.

Storage Instruction: To prevent degradation and avoid repeated freeze-thaw cycles, aliquot your product into small, single-use volumes and store them at -20°C

Application

Immunohistochemistry, Immunocytochemistry, Western Blotting, Glycobiology.