Allium sativum Lectin (ASA) - Texas Red

Allium sativum lectin (ASA) is isolated from garlic bulbs and purified with affinity chromatography. It is a dimer of two subunits. ASA binds to several α1-2-linked mannose residues. The lectin recognizes internal mannose and binds to the core pentasaccharide of N-linked glycans. In addition, the removal of sialic acids enhances binding activity. The binding site can withstand terminal sialic acid and galactose residues. The blood group specificity of ASA is towards rabbit erythrocytes and weak interaction with human erythrocytes. ASA has shown antiproliferative and apoptosis-inducing activity, making it a relative subject in cancer research.

Texas Red is a red-fluorescent dye that, when bound to ASA, effectively reveals the binding pattern in cellular imaging applications. With minimal overlap between its emission spectra and FITC, this combination is ideal for dual-labeling experiments. Additionally, Texas Red offers greater photostability and is less sensitive to pH changes compared to other dyes like fluorescein.

Recommended Usage: Recommended dilution is 0.5 – 10 µg/ml. 1XPBS can be used to dilute the stock. Less than 0.5 ug /ml will agglutinate rabbit erythrocytes.

Storage Instruction: To prevent degradation and avoid repeated freeze-thaw cycles, aliquot your product into small, single-use volumes and store them at -20°C

Application

Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Glycobiology.

References

1. Ghosh, P., Sen, S., Chakraborty, J., & Das, S. (2016). Monitoring the efficacy of utated Allium sativum leaf lectin in transgenic rice against Rhizoctonia solani. BMC biotechnology, 16, 24. https://doi.org/10.1186/s12896-016-0246-0.

2. Kumar, S., Jitendra, K., Singh, K., Kapoor, V., Sinha, M., Xess, I., Das, S. N., Sharma, S., Singh, T. P., & Dey, S. (2015). Biological Properties and Characterization of ASL50 Protein from Aged Allium sativum Bulbs. Applied biochemistry and biotechnology, 176(7), 1914–1927.